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g418 disulfate  (Thermo Fisher)


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    Structured Review

    Thermo Fisher g418 disulfate
    G418 Disulfate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/g418/G418+disulfate%2C+solution/pm42365094-171-8-10
    Average 95 stars, based on 1 article reviews
    g418 disulfate - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    other:

    Article Title: DNA hypomethylating agents preserve T cell stemness and potentiate the efficacy of CD3-bispecific antibodies
    Article Snippet: TrampC2/hPSMA cells were maintained in DME high glucose (Irvine Scientific) with 5% FBS (VWR), 5% Nu Serum IV (VWR), 5 μg/mL of insulin (Gemini Bio-Products), 10 nmol/L dehydroandrosterone (Sigma-Aldrich), and 2 mmol/L L-glutamine (Irvine Scientific), 500 μg/mL of penicillin, 500 μg/mL streptomycin (Thermo Fisher Scientific), and 500 μg/mL G418 (Thermo Fisher Scientific).

    Cell Culture:

    Article Title: Double-stranded oligonucleotide, composition and conjugate comprising double-stranded oligonucleotide, preparation method thereof and use thereof
    Article Snippet: HepG2.2.15 cells were purchased from ATCC and cultured in DMEM complete medium (Gibco) containing 10% fetal bovine serum (FBS, Gibco), 2 mM L-glutamine (Gibco) and 380 μg/ml G418 at 37° C. in an incubator containing 5% CO2/95% air. .. Huh7 cells were purchased from ATCC and cultured in DMEM complete medium (Gibco) containing 10% fetal bovine serum (FBS, Gibco), 2 mM L-glutamine (Gibco) and 380 μg/ml G418 at 37° C. in an incubator containing 5% CO2/95% air. .. Unless otherwise specified, LipofectamineTM2000 (Invitrogen company) was used as a transfection agent when cells were transfected with various synthesized siRNA or siRNA conjugates below.

    Article Title: Scaffold-free 3D-cell co-culture model system for the study of metastatic cancer in the brain TME.
    Article Snippet: HBEC-5i human brain endothelial cells were obtained from ATCC (Manassas, VA). .. Cell culture media [RPMI 1640 (1X), McCoy’s 5A (modified), DMEM high glucose (HG)], fetal bovine serum (FBS), GlutaMAX, Pen-Strep, puromycin, G418, Dulbecco’s phosphate buffered saline (DPBS), trypsin (0.25%)/EDTA (0.53 mM), and propidium iodide (PI) dead cell stain were purchased from ThermoFisher (Carlsbad, CA). .. Lapatinib ditosylate was acquired from Selleck (Houston, TX), and endothelial cell growth supplement (ECGS) and FITC-Dextran from Sigma Aldrich (St. Louis, MO).

    Article Title: Discovery of 4‐Quinazolinone‐Containing Phenylalanine Derivatives as Potent, Resistant‐Tolerant HIV Capsid Inhibitors
    Article Snippet: .. Human astroglioma U87 cells stably expressing CD4 and CXCR4 (obtained from Prof. Hongkui Deng, Peking University, and Prof. Dan Littman, New York University, USA, via the AIDS Research and Reference Reagent Program, Division of AIDS, NIAID, NIH) [ , ] were cultured in DMEM containing 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin, and 2 mM l ‐glutamine, supplemented with 300 μg/mL G418 (Thermo Scientific, Waltham, MA) and 1 μg/mL puromycin (Thermo Scientific). ..

    Selection:

    Article Title: CaMKII promotes BCR-induced apoptosis through Bcl-xL downregulation in immature B cells.
    Article Snippet: .. After an initial 48-hour recovery period, stable transformants were selected by supplementing the medium with 1 mg/mL G418 (Life Technologies, Grand Island, NY), an aminoglycoside antibiotic used for the selection of stably transfected cells expressing the neomycin resistance gene (pSV2neo). ..

    Stable Transfection:

    Article Title: CaMKII promotes BCR-induced apoptosis through Bcl-xL downregulation in immature B cells.
    Article Snippet: .. After an initial 48-hour recovery period, stable transformants were selected by supplementing the medium with 1 mg/mL G418 (Life Technologies, Grand Island, NY), an aminoglycoside antibiotic used for the selection of stably transfected cells expressing the neomycin resistance gene (pSV2neo). ..

    Article Title: Discovery of 4‐Quinazolinone‐Containing Phenylalanine Derivatives as Potent, Resistant‐Tolerant HIV Capsid Inhibitors
    Article Snippet: .. Human astroglioma U87 cells stably expressing CD4 and CXCR4 (obtained from Prof. Hongkui Deng, Peking University, and Prof. Dan Littman, New York University, USA, via the AIDS Research and Reference Reagent Program, Division of AIDS, NIAID, NIH) [ , ] were cultured in DMEM containing 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin, and 2 mM l ‐glutamine, supplemented with 300 μg/mL G418 (Thermo Scientific, Waltham, MA) and 1 μg/mL puromycin (Thermo Scientific). ..

    Transfection:

    Article Title: CaMKII promotes BCR-induced apoptosis through Bcl-xL downregulation in immature B cells.
    Article Snippet: .. After an initial 48-hour recovery period, stable transformants were selected by supplementing the medium with 1 mg/mL G418 (Life Technologies, Grand Island, NY), an aminoglycoside antibiotic used for the selection of stably transfected cells expressing the neomycin resistance gene (pSV2neo). ..

    Expressing:

    Article Title: CaMKII promotes BCR-induced apoptosis through Bcl-xL downregulation in immature B cells.
    Article Snippet: .. After an initial 48-hour recovery period, stable transformants were selected by supplementing the medium with 1 mg/mL G418 (Life Technologies, Grand Island, NY), an aminoglycoside antibiotic used for the selection of stably transfected cells expressing the neomycin resistance gene (pSV2neo). ..

    Article Title: Discovery of 4‐Quinazolinone‐Containing Phenylalanine Derivatives as Potent, Resistant‐Tolerant HIV Capsid Inhibitors
    Article Snippet: .. Human astroglioma U87 cells stably expressing CD4 and CXCR4 (obtained from Prof. Hongkui Deng, Peking University, and Prof. Dan Littman, New York University, USA, via the AIDS Research and Reference Reagent Program, Division of AIDS, NIAID, NIH) [ , ] were cultured in DMEM containing 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin, and 2 mM l ‐glutamine, supplemented with 300 μg/mL G418 (Thermo Scientific, Waltham, MA) and 1 μg/mL puromycin (Thermo Scientific). ..

    Article Title: A yeast-based platform for etoposide production via yatein bioconversion
    Article Snippet: .. Yeast transformants were selected on YPD medium plates (20 g.L −1 of peptone from meat, enzymatic digest, Sigma-Aldrich; 10 g.L −1 of yeast extract, Thermo Scientific Chemicals; and 20 g.L −1 of glucose, Euromedex France) supplemented with G418 (200 μg mL −1 , Gibco) and nourseothricin (100 μg mL −1 , Jena Bioscience), and screened for effective integration of each expression cassette by colony PCR. ..

    Modification:

    Article Title: Scaffold-free 3D-cell co-culture model system for the study of metastatic cancer in the brain TME.
    Article Snippet: HBEC-5i human brain endothelial cells were obtained from ATCC (Manassas, VA). .. Cell culture media [RPMI 1640 (1X), McCoy’s 5A (modified), DMEM high glucose (HG)], fetal bovine serum (FBS), GlutaMAX, Pen-Strep, puromycin, G418, Dulbecco’s phosphate buffered saline (DPBS), trypsin (0.25%)/EDTA (0.53 mM), and propidium iodide (PI) dead cell stain were purchased from ThermoFisher (Carlsbad, CA). .. Lapatinib ditosylate was acquired from Selleck (Houston, TX), and endothelial cell growth supplement (ECGS) and FITC-Dextran from Sigma Aldrich (St. Louis, MO).

    Saline:

    Article Title: Scaffold-free 3D-cell co-culture model system for the study of metastatic cancer in the brain TME.
    Article Snippet: HBEC-5i human brain endothelial cells were obtained from ATCC (Manassas, VA). .. Cell culture media [RPMI 1640 (1X), McCoy’s 5A (modified), DMEM high glucose (HG)], fetal bovine serum (FBS), GlutaMAX, Pen-Strep, puromycin, G418, Dulbecco’s phosphate buffered saline (DPBS), trypsin (0.25%)/EDTA (0.53 mM), and propidium iodide (PI) dead cell stain were purchased from ThermoFisher (Carlsbad, CA). .. Lapatinib ditosylate was acquired from Selleck (Houston, TX), and endothelial cell growth supplement (ECGS) and FITC-Dextran from Sigma Aldrich (St. Louis, MO).

    Staining:

    Article Title: Scaffold-free 3D-cell co-culture model system for the study of metastatic cancer in the brain TME.
    Article Snippet: HBEC-5i human brain endothelial cells were obtained from ATCC (Manassas, VA). .. Cell culture media [RPMI 1640 (1X), McCoy’s 5A (modified), DMEM high glucose (HG)], fetal bovine serum (FBS), GlutaMAX, Pen-Strep, puromycin, G418, Dulbecco’s phosphate buffered saline (DPBS), trypsin (0.25%)/EDTA (0.53 mM), and propidium iodide (PI) dead cell stain were purchased from ThermoFisher (Carlsbad, CA). .. Lapatinib ditosylate was acquired from Selleck (Houston, TX), and endothelial cell growth supplement (ECGS) and FITC-Dextran from Sigma Aldrich (St. Louis, MO).

    Polymerase Chain Reaction:

    Article Title: A yeast-based platform for etoposide production via yatein bioconversion
    Article Snippet: .. Yeast transformants were selected on YPD medium plates (20 g.L −1 of peptone from meat, enzymatic digest, Sigma-Aldrich; 10 g.L −1 of yeast extract, Thermo Scientific Chemicals; and 20 g.L −1 of glucose, Euromedex France) supplemented with G418 (200 μg mL −1 , Gibco) and nourseothricin (100 μg mL −1 , Jena Bioscience), and screened for effective integration of each expression cassette by colony PCR. ..



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